Anti-inflammatory Activity of Atsuete Extract
Atsuete or Annatto (Bixa orellana L.) has been traditionally used in Central and South America to treat a number of ailments, including internal inflammation. This medicinal use has been supported by a laboratory study done in the Department of Biomedical Sciences, Faculty of Medicine and Health Sciences, Universiti Putra Malaysia. Where the anti-inflammatory activity of the aqueous extract of Atsuete was tested in a chemically induced inflammation in rats.
The study concluded that the aqueous extract of Atsuete inhibited the histamine-induced paw edema as compared to a control group. These results confirmed the validity of the use of Atsuete extract in the treatment of inflammatory disorders.
Ref: http://www.ncbi.nlm.nih.gov/pubmed/23410184
Antigonorrhoeal activity of Atsuete
In a study published in the Journal of Ethnopharmacology of October 1995, the Faculty of Chemical Sciences and Pharmacy in Guatemala found that the bark of Bixa orella (Atsuete, annatto) has the ability to inhibit the development of five strains of N. gonorrhoea through the process of standard bacteriological procedures. This study supports the traditional herbal use of Atsuete in the treatment of gonorrhoea, a sexually transmitted disease.
http://www.ncbi.nlm.nih.gov/?term=bixa+orellana+gonorrhoea
Atsuete extract and β-carotene enhances antioxidant status and regulate gene expression in neutrophils of diabetic rats.
The antioxidant activity of Atsuete extract was investigated in Universidade Federal de Ouro Preto, Ouro Preto, Brazil. The study showed that the treatment of Atsuete extract and β-carotene has decreased the reactive oxidative species production and the mRNA levels of p22(phox) and p47(phox) while it increased the mRNA levels of SOD and CAT in neutrophils from diabetic rats. These data suggest that Atsuete extract and β-carotene exerts antioxidant effect via inhibition of expression of the NADPH oxidase subunits and increase expression/activity of antioxidant enzymes. http://www.ncbi.nlm.nih.gov/pubmed/22239725
Hypoglycaemic activity of Bixa orellana (Atsuete).
West Indian folklore uses Bixa orellana (Atsuete) in the treatment of diabetes mellitus. Preliminary studies have shown that a crude annatto seed extract exhibited either glucose lowering or hyperglycaemia-inducing activity depending on how it was further manipulated.
A study done in the Department of Basic Medical Sciences, University of West Indies, Jamaica, investigated this further and has found that Atsuete extract can indeed decrease blood glucose levels in fasting normoglycaemic and streptozotocin-induced diabetic dogs. Likewise, Atsuete seed extract suppressed the postprandial rise in blood glucose after an oral glucose load.
Moreover, the aciote extract also caused an increase in insulin-to-glucose ratio in normal dogs. It was concluded that Bixa orellana (Atsuete) lowered blood glucose by stimulating peripheral utilization of glucose. This data confirmed that Atsuete extract has glucose-lowering activity and might be of pharmacological importance. http://www.ncbi.nlm.nih.gov/pubmed/18773125
Anticancer Activity of Atsuete Pigment (norbixin)
Norbixin, a pigment of Bixa orellana (Atsuete) was believed to possess antioxidant potential being a carotenoid. This property has been examined in the Instituto de Biologia Roberto Alcântara Gomes, Universidade do Estado do Rio de Janeiro and has identified that Norbixin is an unusual dicarboxylic water-soluble carotenoid present as a component in the pericarp of the seeds of Bixa orellana L.
Norbixin on the response of Escherichia coli cells to DNA damage induced by UV radiation, hydrogen peroxide (H2O2) and superoxide anions (O2*-)) has protective activity against these agents. It was also found that that norbixin has antimutagenic properties, with a maximum inhibition of H2O2-induced mutagenic activity of 87%, based on the Salmonella mutagenicity test. These results suggests that Atsuete pigment (norbixin) has effectively quenched free radicals and may be of valuable pharmacological application against cancer. http://www.ncbi.nlm.nih.gov/pubmed/15841440